Journal: Biomolecules

Article Title: Incorporation of α2-Plasmin Inhibitor into Fibrin Clots and Its Association with the Clinical Outcome of Acute Ischemic Stroke Patients

doi: 10.3390/biom11030347

Figure Lengend Snippet: ( A ) Effect of thrombin concentration on the incorporation of α2-plasmin inhibitor (α2-PI) into fibrin clots.; ( B ) time dependence of α2-PI incorporation into fibrin clot. Dots and whiskers indicate mean and standard deviation of the results of 10 parallel experiments.

Article Snippet: Proteins were transferred to a PVDF membrane (Bio-Rad, Hercules, CA, USA) and immunostained with polyclonal anti-α2-PI antibody (GA2AP-AP, Affinity Biologicals, ON, Canada) that was labeled with horseradish-peroxidase (HRP) and visualized using enhanced chemiluminescence detection (ECL, Thermo Fisher Scientific, Waltham, MA, USA) on X-ray film (GE Healthcare, Chicago, IL, USA).

Techniques: Concentration Assay, Standard Deviation

Journal: Biomolecules

Article Title: Incorporation of α2-Plasmin Inhibitor into Fibrin Clots and Its Association with the Clinical Outcome of Acute Ischemic Stroke Patients

doi: 10.3390/biom11030347

Figure Lengend Snippet: Effect of plasma FXIII level on the amount of α2-PI incorporated into the fibrin clot. A FXIII-deficient plasma sample (α2-PI = 62 mg/L) was supplemented with different amounts of purified FXIII and clotted by thrombin and Ca 2+ . ( A ) Differences of α2-PI antigen values between the substituted plasma and the respective serum were calculated and incorporation was presented as a percentage of the respective plasma value. ( B ) Fibrin clots were washed, dissolved and analyzed by SDS PAGE and Western blot using horseradish-peroxidase (HRP)-labeled polyclonal anti-α2-PI antibody and enhanced chemiluminescence (ECL) detection.

Article Snippet: Proteins were transferred to a PVDF membrane (Bio-Rad, Hercules, CA, USA) and immunostained with polyclonal anti-α2-PI antibody (GA2AP-AP, Affinity Biologicals, ON, Canada) that was labeled with horseradish-peroxidase (HRP) and visualized using enhanced chemiluminescence detection (ECL, Thermo Fisher Scientific, Waltham, MA, USA) on X-ray film (GE Healthcare, Chicago, IL, USA).

Techniques: Clinical Proteomics, Purification, SDS Page, Western Blot, Labeling